9/9/2023 0 Comments El nucleo movie![]() ![]() All samples have similar profiles indicating a negligible 5-prime bias with high levels of coverage from 5-prime to 3-prime.įigure S7. (C) Localization of aligned reads across a theoretical 100bp gene body. The plateau at higher levels of sampling indicate that all samples were sequenced with enough depth to assay all expressed genes. (B) Junction saturation curves for all samples across all Ensembl known exon-exon junctions randomly sampled from aligned BAM files in 5% intervals. All samples show that greater than 95% of all aligned reads aligned to protein-coding genes. (A) The summed counts-per-million of all Ensembl gene biotypes for every sample. Primary roots of 8d-old DR5-GUS ( Ulmasov et al., 1997) seedlings that were treated with the indicated concentration of IAA prior to staining to visualize GUS activity.įigure S6. Auxin induction of DR5:GUS activity in root tissues track in figure 3A, but provides quantification of the values.įigure S5. ![]() Although there is a drop towards the C-terminus of the prion-like domain, the majority of the region shows a strong tendency to undergo self-interaction. Each value uses an average sliding-window of the three surrounding fragments to capture chain connectivity. (B) The per-fragment average attractive interaction, written as an aggregation propensity. Attractive interaction reflects the affinity between two fragments, with any value greater than 0 reflecting a higher affinity than would expected for two fragments that did not experience any attractive interactions. (A) The interaction matrix generated by simulations of all unique pairs of non-overlapping six residue fragments taken from the ARF19 prion-like domain. Figure generated by the PLAAC webserver ( ).įigure S4. The prion-like domain is highlighted in yellow. Results of ARF19 analysis by the PLAAC algorithm. Identification of a prion-like domain in ARF19 Across the 21123 discrete disordered regions the ARF19 prion-like domain was among the least charged (bottom 2%) and most enriched in the combined fraction of glutamine (Q), serine (S), asparagine (N) and leucine (L) (above 99.7% of all other IDRs).įigure S3. To quantitatively assess the sequence composition of the ARF19 PLD we compared it against all predicted disordered regions in the A. Comparative analysis of ARF19 prion-like domain (B) Fluorescence microscopy image from the upper root regions of a 5d-old Arabdopsis seedling expressing UBQ10:YFP-ARF19MR. (A) Confocal microscopy images of a 5d-old seedling expressing pARF7:ARF7g-Venus. ![]()
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